| 11 th Annual Pediatric Critical Care Colloquium |
| Session/Time | Neurology/Fri, 10:00 - 12:00 PM | |||||||||||||||
| Title | Mitogen-Activated Protein (NLAP) Kinase Activity During and After Transient Focal Cerebral Ischemia in Rats | |||||||||||||||
| Author | M Nares, J Kuluz, J Neary, Y Kang, E Xu, CL Schleien | |||||||||||||||
| Affiliation | Department of Pediatrics and VA Medical Center, University of Miami School of Medicine, Miami, FL | |||||||||||||||
| Introduction | MAP kinase plays a critical role in signal transductioa after stimulation of membrane receptors by neurotrophic factors. To investigate the role of this enzyme in stroke, we measured MA.P kinase activity in the brain during and after transient middle cerebral artery occlusion (MCAO) in rats | |||||||||||||||
| Method | Fasted, adult male Sprague Dawley rats were anesthetized with isoflurane 2-3 7o, N20 70 % and O., for induction of MCAO by the intraluminal suture method. Rats were allowed to awaken du'ring MCAO. Rats were sacrificed after 30 min (a=.5) or 2h (n=4) of'NICAO without reperfusion, and 30 min (n=5) or 60 min (n=4') after 2h MCAO. Brains were perfused with cold saline for 2 min and samples of cortex and subcortex from ischemic and non-ischemic henaispheres were assayed for MAP kinase activity (rate of phosphorylation of myelin basic protein). Data (mean ± SEM) analyzed by Student's t-test (* p < .05)<. 05). | |||||||||||||||
| Result | MAP kinase activity was
significantly higher in the ischeiiu'c cortex compared to non-ischeraic cortex 30 min
after 2h MCAO (ratio of ischemic: non-ischemic brain):
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| Conclusion | These findings indicate that cerebral MAP kinase is activated in a region-specific manner during early reperfusion after focal brain ischernia. This correlates temporally with the expression of neurotrophic actors during early reperfusion. (Supported in part by the Department of Veterans Affairs) |
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